Journal: The Journal of Cell Biology

Article Title: Different modes of hypertrophy in skeletal muscle fibers

doi: 10.1083/jcb.200105147

Figure Lengend Snippet: Trend in fiber type changes in gracilis anterior and posterior muscle influenced by exercise not transgene. (A) Gracilis anterior muscle showed a trend of increasing type IIB MyHC at the cost of type IIA/X MyHC–positive fibers. A significant difference was only found in WT-EX and transgenic mice. (B) Fiber type changes in gracilis posterior muscle showed a trend toward type IIA/X MyHC–positive fibers at the cost of fibers expressing type IIB and type I MyHC. Exercise appears to be the main influence on these trends.

Article Snippet: The sections were incubated with either anti-MyHC I antibody (American Type Culture Collection A4.951; 1:10 dilution in PBS/1% BSA) or anti-MyHC IIB antibody (American Type Culture Collection BF-F3; 1:10 dilution in PBS/1% BSA), washed three times in PBS, and incubated in secondary antibody (Jackson ImmunoResearch Laboratory; peroxidase-conjugated goat anti–mouse IgG 115-035-164, or peroxidase-conjugated goat anti–mouse IgM 115-035-075, both 1:500 in PBS/1% BSA).

Techniques: Transgenic Assay, Expressing

Journal: Developmental biology

Article Title: Formation and contraction of multicellular actomyosin cables facilitate lens placode invagination

doi: 10.1016/j.ydbio.2020.02.014

Figure Lengend Snippet: Highlights:

Article Snippet: Whole-mount embryo, immunolabeling and imaging Fixed, whole mouse embryos isolated from time-mated females (E9.75–E10.5) or chicken embryos were rinsed three times in 0,1% Triton X100-PBS and placed into 4% milk (in 0,1% Triton X100-PBS) containing one or more of the following primary antibodies or fluorescent labels overnight at 4°C with constant rocking: rabbit-anti-β-catenin (1:500; Santa Cruz, sc-7199), mouse anti-β-catenin (1:500; BD Biosciences, 610153), rabbit anti-myosin IIb (1:500; Covance, PRB445P), mouse anti-myosin IIB (1:250; CMII 23-s, DSHB), rabbit Par3 (1:500; Millipore, #07–330), rabbit anti-Arhgef11 (1:200, Atlas Antibodies, HPA01126), rat anti- Phospho-Myosin Light Chain 2 (Ser19) (1:250, Cell Signaling Technology, 3675S), Phalloidin 488 (1:500; Invitrogen, A12379), rabbit anti-Prox1(1:1K, EMD Millipore, AB5475), and Hoechst 33342 (1:1000; Sigma, B-2261).

Techniques: Plasmid Preparation, Recombinant, Software